| Catalog # | I1903-13B |
| Applications | Suitable for Immunomicroscopy, Immunofluorescence, Flow Cytometry and FACS Analysis as well as other antibody based uorescent assays. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:100-1:200 (~1ug PE conjugate will stain 1x10e6 cells). |
| IF Microscopy: 1:100-1:200 |
| Optimal dilutions to be determined by the researcher. |
| Label | R-Phycoerythrin (PE), 0.5mg/ml (determined by Abs: 82.0 at 565nm for a 1% solution for only those molecules to which at least one molecule of active antibody is bound). |
| MW of Label | 240kD |
| Absorption Wavelength | 490, 545, 565nm |
| Emission Wavelength | 580nm |
| OD566nm/OD280nm | 2.6 |
| Note | Concentration value is Phycobiliprotein concentration. It is determined by absorbance = 82.0 at 565nm for a 1% solution for only those R-PE molecules to which one molecule of active antibody is bound. |
| Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light and cold. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | F(ab’)2 |
| Host | Donkey |
| Source | Rabbit |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with R-Phycoerythrin (PE). |
| Purity | Purified by immunoaf nity chromatography. |
| Immunogen | Rabbit IgG whole molecule |
| Specificity | Recognizes rabbit IgG heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-donkey serum, rabbit IgG and rabbit serum. No reaction was observed against anti-pepsin, anti-donkey IgG F(c) or to bovine, chicken, goat, guinea pig, hamster, equine, human, mouse, rat and sheep serum proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
