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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG (H&L) (PE), X-Adsorbed

Anti -IgG (H&L) (PE), X-Adsorbed

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Specifications

Clone Host Grade Applications
Polyclonal Donkey Affinity Purified FC IF
Catalog #I1903-13B
ApplicationsSuitable for Immunomicroscopy, Immunofluorescence, Flow Cytometry and FACS Analysis as well as other antibody based uorescent assays. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:100-1:200 (~1ug PE conjugate will stain 1x10e6 cells).
IF Microscopy: 1:100-1:200
Optimal dilutions to be determined by the researcher.
LabelR-Phycoerythrin (PE), 0.5mg/ml (determined by Abs: 82.0 at 565nm for a 1% solution for only those molecules to which at least one molecule of active antibody is bound).
MW of Label240kD
Absorption Wavelength490, 545, 565nm
Emission Wavelength580nm
OD566nm/OD280nm2.6
NoteConcentration value is Phycobiliprotein concentration. It is determined by absorbance = 82.0 at 565nm for a 1% solution for only those R-PE molecules to which one molecule of active antibody is bound.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light and cold.
Clone TypePolyclonal
IsotypeF(abí)2
HostDonkey
SourceRabbit
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with R-Phycoerythrin (PE).
PurityPurified by immunoaf nity chromatography.
ImmunogenRabbit IgG whole molecule
SpecificityRecognizes rabbit IgG heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-donkey serum, rabbit IgG and rabbit serum. No reaction was observed against anti-pepsin, anti-donkey IgG F(c) or to bovine, chicken, goat, guinea pig, hamster, equine, human, mouse, rat and sheep serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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