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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG H&L, X-adsorbed (PE)

Anti -IgG H&L, X-adsorbed (PE)

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Specifications

Clone Host Grade Applications
Polyclonal Donkey Affinity Purified FC IF
Catalog #I1903-13U
ApplicationsSuitable for use in Immunomicroscopy, Flow Cytometry or FACS and Immunofluorescence. Other applications not tested.
Recommended DilutionsImmunomicroscopy: 1:100-1:250
Flow Cytometry: 1:100-1:250 Maximum amount of reagent required to stain 1x10e6 cells is ~1.0ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Immunofluorescence: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Excitation Wavelength 488nm
Emission Wavelength: 575nm
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostDonkey
SourceGoat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.02% sodium azide. Labeled with R-Phycoerythrin (R-PE).
PurityPurified by immunoaffinity chromatography.
ImmunogenGoat IgG whole molecule
SpecificityRecognizes goat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-donkey serum, goat IgG and goat serum. No reaction was observed against chicken, guinea pig, hamster, equine, mouse, human, rabbit or rat serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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