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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG, H&L (HRP)

Anti -IgG, H&L (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
Catalog #I1903-14R
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1: 5000 -1:50,000, assayed against 1.0ug of Rat IgG in a standard capture using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
Immunohistochemistry: 1:500-1:2500
Western Blot: 1:1000-1:10,000
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceRat
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with HRP.
PurityPurified by immunoaffinity chromatography.
ImmunogenRat IgG whole molecule
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Rat IgG and Rat Serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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