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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B IH Gel

Catalog #	I1903-14R
Applications	Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1: 5000 -1:50,000, assayed against 1.0ug of Rat IgG in a standard capture using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
	Immunohistochemistry: 1:500-1:2500
	Western Blot: 1:1000-1:10,000
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Rat
Concentration	~2mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with HRP.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Rat IgG whole molecule
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Rat IgG and Rat Serum.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.