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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (AP)

Anti -IgG, Fc (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1903-17D
ApplicationsSuitable for use in Immunoblot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:3000-1:44,000. 1.0ug of Canine IgG was used in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C as an undiluted liquid for 12 months. Dilute only prior to immediate use. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceCanine
Concentration~0.7mg/ml
FormSupplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Label: Alkaline Phosphatase (Calf Intestine) (MW 140,000 daltons)
PurityPurified by immunoaffinity chromatography.
ImmunogenCanine IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Canine IgG, Canine IgG F(c) and Canine Serum. No reaction was observed against Canine IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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