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Anti -IgG, Fc (AP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| Catalog # | I1903-17D | | Applications | Suitable for use in Immunoblot, ELISA and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:3000-1:44,000. 1.0ug of Canine IgG was used in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C as an undiluted liquid for 12 months. Dilute only prior to immediate use. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Canine | | Concentration | ~0.7mg/ml | | Form | Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Label: Alkaline Phosphatase (Calf Intestine) (MW 140,000 daltons) | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Canine IgG F(c) fragment | | Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Canine IgG, Canine IgG F(c) and Canine Serum. No reaction was observed against Canine IgG F(ab’)2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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