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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

Catalog #	I1903-17H
Applications	Suitable for use in ELISA, Immunofluorescence Microscopy and fluorescent Western Blot. Also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Other applications not tested.
Recommended Dilution	ELISA: 1:10,000-1:50,000
	Immunofluorescence Microscopy: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Absorption Wavelength	596nm
Emission Wavelength	620nm
Fluorochrome/Protein Ratio	2.4 moles Texas Red per mole of rabbit IgG
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Texas Red conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Canine
Concentration	~2mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Texas Red (MW 625 daltons).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Canine IgG F(ab’)2 fragment
Specificity	Recognizes canine IgG F(ab’)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, canine IgG, canine IgG F(ab’)2 and canine serum. No reaction was observed against canine IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.