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Anti -IgG, Fc (HRP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH Gel |
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| Catalog # | I1903-19K | | Applications | Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:20,000-1:200,000. Use 1ug of equine IgG in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT. | | Optimal dilutions should be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Equine | | Concentration | ~1.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with Horseradish Peroxidase (HRP). | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Equine IgG F(c) fragment | | Specificity | Recognizes equine IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, equine IgG, equine IgG F(c) and equine serum. No reaction was observed against equine IgG F(ab’)2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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