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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (HRP)

Anti -IgG, Fc (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1903-19K
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:20,000-1:200,000. Use 1ug of equine IgG in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT.
Optimal dilutions should be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceEquine
Concentration~1.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with Horseradish Peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenEquine IgG F(c) fragment
SpecificityRecognizes equine IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, equine IgG, equine IgG F(c) and equine serum. No reaction was observed against equine IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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