Applications
Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blotting: 1:1000-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against source serum protein and IgG.
Concentration
~1mg/ml (UV 280)
Form
Lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Conjugated to horseradish peroxidase (HRP).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.