| Catalog # | I1903-21T |
| Applications | Suitable for use in immunoblot, ELISA, immunohistochemistry, immunomicroscopy. Other applications not tested. |
| Recommended Dilution | ELISA (standard capture): 1:3,000-1:15,000, 1.0 ug of Horse IgG using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. |
| Optimal dilution determined by the researcher. |
| Label | Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) |
| Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Equine |
| Concentration | ~ 1.5 mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA,(IgG and protease free), 0.01% sodium azide, 40% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | HorseIgG F(ab’)2 fragment |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti- |
| Rabbit Serum, Horse IgG, Horse IgG F(ab’)2 and Horse Serum. No reaction was observed against Horse IgG |
| F(c). |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
