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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG, F(abí)2 (HRP)

Anti -IgG, F(abí)2 (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1903-21V
ApplicationsSuitable for immunoblot (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry. Other applications not tested.
Recommended DilutionELISA (standard capture): 1:15,000-1:75,000, 1.0 ug of Horse IgG using ABTS (2,2í-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceEquine
Concentration~ 1.5 mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA,(IgG and protease free), 0.01% Gentamicin Sulfate, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenHorse IgG F(abí)2 fragment
LabelHorseradish Peroxidase (HRP)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG, Horse IgG F(abí)2 and Horse Serum. No reaction was observed
against Horse IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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