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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH Gel

Catalog #	I1903-21V
Applications	Suitable for immunoblot (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA (standard capture): 1:15,000-1:75,000, 1.0 ug of Horse IgG using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Equine
Concentration	~ 1.5 mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA,(IgG and protease free), 0.01% Gentamicin Sulfate, 40% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Horse IgG F(ab’)2 fragment
Label	Horseradish Peroxidase (HRP)
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG, Horse IgG F(ab’)2 and Horse Serum. No reaction was observed
against Horse IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.