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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG, F(abí)2 (AP)

Anti -IgG, F(abí)2 (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1903-21W
ApplicationsSuitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry. Other applications not tested.
Recommended DilutionELISA (standard capture): 1:1000-1:4000, 1.0 ug of Horse IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceEquine
Concentration~1.0 mg/ml
FormSupplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% Glycerol; pH 8.0, 10 mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenHorse IgG F(abí)2 fragment Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Horse IgG, Horse IgG F(abí)2 and Horse Serum. No reaction was observed against Horse IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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