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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH Gel

Catalog #	I1903-21Y
Applications	Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA (standard capture): 1:1000 to 1:5,000, 1.0 ug of Horse IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1.0 mg/ml
Form	Supplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% Glycerol; pH 8.0 0.01% Sodium Azide, 10 mg/ml BSA (IgG and Protease free)
Purity	Purified by immunoaffinity chromatography.
Immunogen	Horse IgG F(ab’)2 fragment
Label	Alkaline Phosphatase (Calf Intestine) (MW)140kD)
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline
Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F(ab’) 2 and Horse Serum. No reaction was observed against Horse IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.