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Specifications

Clone Host Grade Applications Polyclonal Donkey Affinity Purified E B IH

Catalog #	I1903-28
Applications	Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilutions	ELISA: 1:8300
	Western Blot: 1:500-1:2500
	Immunohistochemistry: 1:200-1:1000
	Optimal dilutions to be determined by the researcher.
Substrate	N2600-10 RT for 30 minutes.
Label	Alkaline phoshpatase (calf intestine) (MW 14kD)
Storage and Stability	May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Donkey
Source	Goat
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Labeled with Alkaline Phoshpatase (AP).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Goat IgG whole molecule.
Specificity	Recognizes goat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-donkey serum, goat IgG and goat serum.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.