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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG (AP)

Anti -IgG (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Donkey Affinity Purified E B IH
Catalog #I1903-28
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended DilutionsELISA: 1:8300
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Substrate N2600-10 RT for 30 minutes.
LabelAlkaline phoshpatase (calf intestine) (MW 14kD)
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Clone TypePolyclonal
IsotypeIgG
HostDonkey
SourceGoat
Concentration~1mg/ml
FormSupplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Labeled with Alkaline Phoshpatase (AP).
PurityPurified by immunoaffinity chromatography.
ImmunogenGoat IgG whole molecule.
SpecificityRecognizes goat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-donkey serum, goat IgG and goat serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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