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You are here:Home » Antibodies » Antibodies-IgG Conjugates » Anti -IgG, Fab, H&L (FITC)

Anti -IgG, Fab, H&L (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified FC IF
Catalog #I1903-31H
ApplicationsSuitable for use in FLISA, Flow Cytometry and Immunofluorescence. Other applications not tested.
Recommended DilutionFLISA: 1:10,000 - 1:50,000
Immunofluorescence: 1:1,000 - 1:5,000
Flow Cytometry: 1:500 - 1:2,500
Optimal dilutions to be determined by the researcher.
Absorption Wavelength 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 1.4 moles FITC per mole of Rabbit IgG Fab
Storage and StabilityLyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. 
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG,Fab,H&L
HostRabbit
SourceGuinea pig
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label
PurityPurified by immunoaffinity chromatography.
ImmunogenGuinea Pig IgG whole molecule
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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