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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1903-32A
Applications	Suitable for use in Immunoblot, ELISA, Immunomicroscopy and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:28,000-1:114,000 Streptavidin (HRP) (S7973-97A) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6sulfonic acid]) as a substrate for 30 minutes at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Guinea pig
Concentration	~2mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio	10-20 BAC molecules per Goat IgG molecule
Purity	Purified by immunoaffinity chromatography.
Immunogen	Guinea Pig IgG F(ab’)2 fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, guinea pig IgG, guinea pig IgG F(ab’)2 and guinea pig Serum. No reaction was observed against guinea pig IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.