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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (Biotin)

Anti -IgG, Fc (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1903-32B
ApplicationsSuitable for use in Immunoblot, ELISA, immunomicroscopy and immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:4000-1:20,000; Streptavidin (HRP) (S7973-97A) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6sulfonic acid]) as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceGuinea pig
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio10-20 BAC molecules per Goat IgG molecule
PurityPurified by immunoaffinity chromatography.
ImmunogenGuinea Pig IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin,
anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(ab’)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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