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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG, H&L, X-adsorbed (HRP)

Anti -IgG, H&L, X-adsorbed (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Donkey Affinity Purified E B IH
Catalog #I1903-35X
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:10,000-1:50,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostDonkey
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with HRP. Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgG whole molecule.
SpecificityRecognizes human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-donkey serum, human IgG and human serum. No reaction was observed against Bovine, Chicken, Goat, guinea pig, Hamster, equine, Mouse, Rabbit, Rat and Sheep Serum Proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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