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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1903-36B
Applications	Suitable for use in Western Blot, ELISA and immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:2000-1:10,000
	Western Blot: 1:500-1:2500
	Immunohistochemistry: 1:200-1:1000
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~ 0.5mg/ml
Form	Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, pH 8.0, 0.001M magnesium chloride, 0.0001M zinc chloride, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Labeled with Alkaline phophatade (AP).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human IgG, whole molecule
Specificity	Recognizes human IgG H&L. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgG and human serum. No reaction was observed against bovine, chicken, goat, guinea pig, hamster, equine, mouse, rabbit, rat and sheep serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.