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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG (H&L), X-adsorbed (AP)

Anti -IgG (H&L), X-adsorbed (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1903-36B
ApplicationsSuitable for use in Western Blot, ELISA and immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:2000-1:10,000
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~ 0.5mg/ml
FormSupplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, pH 8.0, 0.001M magnesium chloride, 0.0001M zinc chloride, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Labeled with Alkaline phophatade (AP).
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgG, whole molecule
SpecificityRecognizes human IgG H&L. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgG and human serum. No reaction was observed against bovine, chicken, goat, guinea pig, hamster, equine, mouse, rabbit, rat and sheep serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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