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You are here:Home » Antibodies » Abs to IgG Kappa » Anti -IgG, Kappa (Biotin)

Anti -IgG, Kappa (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1903-36D
ApplicationsSuitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:2000-1:10,000
ELISA: 1:15,000-1:75,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
LabelBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio10-20 BAC molecules per goat IgG molecule
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Conjugated to biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman kappa chain
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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