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You are here:Home » Antibodies » Abs to IgG Kappa » Anti -IgG, Kappa (TRITC)

Anti -IgG, Kappa (TRITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IF
Catalog #I1903-36S
ApplicationsSuitable for use in ELISA and Immunofluorescence. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
LabelTetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength 550nm
Emission Wavelength 570nm
Fluorochrome/Protein Ratio 4.0 moles TRITC per mole of Goat IgG F(abí)2
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Sodium Azide. Labeled with TRITC.
PurityPurified by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
ImmunogenHuman (kappa chain)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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