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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1903-37P
Applications	Suitable for use in Western Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	ELISA: 1:50,000
	Western Blot: 1:2000-1:10,000
	Immunohistochemistry: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Human
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% thimerosal. Labeled with HRP. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human kappa chain.
Specificity	Recognizes human IgG,k. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-goat serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.