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You are here:Home » Antibodies » Abs to IgG Kappa » Anti -IgG (kappa chain specific) (AP)

Anti -IgG (kappa chain specific) (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1903-37Q
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
LabelAlkaline Phosphatase (Calf Intestine), M.W.-140,000D
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Alkaline Phosphatase.
PurityPurified by Immunoaffinity chromatography.
ImmunogenHuman kappa chain
SpecificityRecognizes human IgG (kappa chain specific). No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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