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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH Gel

Catalog #	I1903-37Q
Applications	Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	ELISA: 1:200-1:1000
	Optimal dilutions to be determined by the researcher.
Label	Alkaline Phosphatase (Calf Intestine), M.W.-140,000D
Storage and Stability	May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
	Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Alkaline Phosphatase.
Purity	Purified by Immunoaffinity chromatography.
Immunogen	Human kappa chain
Specificity	Recognizes human IgG (kappa chain specific). No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.