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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -IgG, lambda chain (HRP)

Anti -IgG, lambda chain (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1903-37T
ApplicationsSuitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron
microscopy and immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:200-1:1000 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceHuman
FormSupplied as a lyophilized powder from 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with horseradish peroxidase (HRP) (MW:40kD).
PurityPurified by affinity chromatography.
ImmunogenHuman lambda chain
SpecificityAssay by IEP resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No
reaction was observed against anti-Pepsin or anti-Goat IgG F(c). <1% cross reactivity against other human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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