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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH Gel

Catalog #	I1903-38H
Applications	Suitable for Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	ELISA: 1:1600-1:6250
	Optimal dilutions to be determined by the researcher.
Label	Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Human
Concentration	0.8 mg/ml (by UV absorbance at 280 nm)
Form	Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Sodium Azide
Purity	Purified by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Immunogen	Human IgG F(c) fragment
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab’)2 or Bovine, Horse, Mouse and Rat Serum Proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.