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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc, X-adsorbed (AP)

Anti -IgG, Fc, X-adsorbed (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1903-38H
ApplicationsSuitable for Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:1600-1:6250
Optimal dilutions to be determined by the researcher.
LabelAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceHuman
Concentration0.8 mg/ml (by UV absorbance at 280 nm)
FormSupplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Sodium Azide
PurityPurified by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
ImmunogenHuman IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(abí)2 or Bovine, Horse, Mouse and Rat Serum Proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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