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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG, Fab, H&L (FITC)

Anti -IgG, Fab, H&L (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Catalog #I1903-38R
ApplicationsSuitable for use in FLISA and Immunofluorescence. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
LabelFITC MW: 390D
Absorption Wavelength 495 nm
Emission Wavelength 528 nm
Fluorochrome/Protein Ratio 1.3 moles FITC per mole of Rabbit IgG Fab
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration0.5 mg/ml (by UV absorbance at 280 nm)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Sodium Azide
PurityPurified by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
ImmunogenHuman IgG whole molecule
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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