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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -IgG F(abí)2 (lambda chain specific) (AP)

Anti -IgG F(abí)2 (lambda chain specific) (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1903-38W
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:200 to 1:1000. 1.0ug of Human IgG was used in a standard capture ELISA with pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M Zznc chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Labeled with alkaline phosphatase (AP).
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman lambda chain. MW of label: 140kD
SpecificityRecognizes human lambda light chain. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-goat serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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