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Anti -IgG F(ab’)2 (lambda chain specific) (AP)
Anti -IgG F(ab’)2 (lambda chain specific) (AP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| Catalog # | I1903-38W | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:200 to 1:1000. 1.0ug of Human IgG was used in a standard capture ELISA with pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | F(ab’)2 | | Host | Goat | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M Zznc chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Labeled with alkaline phosphatase (AP). | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Human lambda chain. MW of label: 140kD | | Specificity | Recognizes human lambda light chain. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-goat serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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