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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (HRP)

Anti -IgG, Fc (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit E B IH
Catalog #I1903-38Y
ApplicationsSuitable for use in Immunoblot (Western or dot blot), ELISA, Immunohistochemistry and Immunoperoxidase electron microscopy. Other applications not tested.
Recommended DilutionELISA: 1:2000 to 1:8000, Use ABTS as a substrate for 30 min at RT to assay against 1ug of human IgG. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~2mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.4,10mg/ml BSA (IgG and protease free), and 0.01% gentamicin sulfate, before the addition of glycerol to 40%. Labeled with horseradish peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgG F(c) fragment
SpecificityRecognizes human IgG Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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