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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E IH

Catalog #	I1903-50K
Applications	Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:200-1:5000
	Immunohistochemistry: 1:500-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.1% kathon CG. Labeled with horseradish peroxidase (HRP). Reconstitute with 1.1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Purified human IgE heavy chain
Specificity	Recognizes heavy chain of human IgE. No reactivity has been observed with non-IgE human serum immunogloublins and with light chains on all human immunoglobulins. Does not react with serum proteins from bovine, mouse or rabbit
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.