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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (FITC)

Anti -IgG, Fc (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B FC IF
Catalog #I1903-58
ApplicationsSuitable for use in FLISA (Fluorescent), Flow Cytometry and Immunofluorescence. Other applications not tested.
Recommended DilutionFLISA (Fluorescent): 1:10,000-:50,000
IF Microscopy: 1:1,000-1:5,000
Flow Cytometry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.
Fluorochrome/Protein Ratio2.6 moles FITC per mole of goat IgG F(abí)2
Absorption Wavelength 495nm
Emission Wavelength 528nm
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(ab')2
HostGoat
SourceHuman
Concentration~1mg/ml (UV280nm)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Labeled with FITC (MW390D). Reconstitution: Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long term storage.
PurityPrepared by affinity chromatography.
ImmunogenHuman IgG F(c) fragment.
SpecificityRecognizes Human IgG F(c) fragment. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), human IgG F(abí)2 or bovine, equine, mouse, rabbit, rat and sheep serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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