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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1903-58F1
Applications	Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested.
Recommended Dilution	ELISA: 1:20,000 to 1:400,000
	Western Blot: 1:20,000-1:400,00
	Immunohistochemistry (tissue sections): 1:500-1:5000
	Optimal dilutions to be determined by the researcher.
Label	Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio	10-20 BAC molecules per Goat IgG molecule
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Human
Concentration	1.2 mg/ml (by UV absorbance at 280 nm)
Form	Supplied as a lyophilized powder from PBS, pH 7.6, 15mg/ml BSA (IgG and Protease free), 0.01% (w/v) Sodium Azide, 0.01% (w/v) Thimerosal.
Purity	Purified by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Immunogen	Human IgA a heavy chain
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.