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You are here:Home » Antibodies » Abs to IgA » Anti -IgA, alpha (HRP)

Anti -IgA, alpha (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1903-58G
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:5000-1:100,000
ELISA: 1:5000-1:100,000
Immunohistochemistry: 1:500-1:5000
Optimal dilutions to be determined by the researcher.
LabelHorseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Thimerosal. Do not add azide!
PurityPurified by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
ImmunogenHuman alpha heavy chain
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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