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You are here:Home » Antibodies » Abs to IgG IgA IgM » Anti -IgG, IgA, IgM, H&L, X-adsorbed (HRP)

Anti -IgG, IgA, IgM, H&L, X-adsorbed (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1903-58G2
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:2000-1:50,000 using ABTS (Cat# A0030) as a substrate for 30 minutes at RT.
Western Blot:1:1000-1:10,000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 500ul sterile ddH2O.
PurityPurified by immunoaffinity chromatography
ImmunogenHuman IgG, IgA, and IgM whole molecules.
SpecificityRecognizes human IgG, IgA and IgM (H&L), (all Human immunoglobulin classes, isotypes and chain combinations). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-goat serum. No reaction was detected against anti-goat IgG F(c), or mouse serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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