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You are here:Home » Antibodies » Abs to IgG IgA IgM » Anti -IgG, IgA, IgM, H&L, X-adsorbed (FITC)

Anti -IgG, IgA, IgM, H&L, X-adsorbed (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IF
Catalog #I1903-58G6
ApplicationsSuitable for use in ELISA (Fluorescent), Fluorescent Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:500-1:2500
Optimal dilutions to be determined by the researcher.
Absorption Wavelength 495nm
Emission Wavelength 528nm
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceMouse
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgG, IgA, and IgM whole molecules
SpecificityRecognizes mouse IgG, IgA, and IgM (all isotypes and chain combinations). Assay by IEP resulted in a single precipitin arc against anti-fluorescein and anti-goat serum. Does not crossreact with anti-goat IgG F(c), anti-pepsin or human serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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