Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

ELISA: 1:250,000 Westerrn Blot: 1:2000-1:20,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Mouse IgG whole molecule.

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA |(IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.

Purified by immunoaffinity chromatography.

Recognizes mouse IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, mouse IgG and mouse serum. No reaction was observed against bovine, chicken, goat, guinea pig, hamster, equine, human, rabbit, rat and sheep serum proteins.