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Specifications

Many monoclonal antibodies of animal origin are valuable diagnostic agents. Their therapeutic use, however, has been frequently limited by hypersensitivity reactions or by anti-immunglobulin
responses. The replacement of the animal with human antibodies solves most of these problems, but the production of human monoclonal antibodies by classical hybridoma techniques or EBV transformation is limited by the instability of cell lines, low antibody yields and the ethic limitations of immunizing humans with antigens. A promising alternative to the hybridoma technology is the production of recombinant antibodies. Pioneering work of the last decade showed that it is possible to amplify rearranged immunoglobulin genes from Blymphocytes, to insert them into different vectors, and to express them in bacteria, in yeast, in insect, mammalian or plant cells. Moreover, the randomized combination of cloned heavy and light chain immunoglobulin gene fragments allowed the construction of human antibody libraries. These libraries enable today the isolation of specific antibodies against particular antigens by phage display techniques. This screening strategy offers the possibility to obtain specific human antibodies without prior immunization of a lymphocyte donor. One prerequisite for generating highly diversified human antibody libraries, however, is the development of PCR primers capable of amplifying all rearranged immunglobulin genes. Previous primer designs have been based on nucleotide sequences of immunglobulin genes. PROGEN offers a primer set based on amino acid sequences of antibodies, which allows the amplification of rearranged human immunglobulin genes of individual B cell clones as well as of larger B cell populations for the construction of human scFvantibody libraries.
Catalog #	I1904-10
	PCR amplification (Set 1) and cloning (Set 2) of human IgG and IgM heavy and light chain variable domain coding regions.
	Application Generation of large repertoires of rearranged immunoglobulin variable domain coding regions for the construction of human IgG and IgM scFv-antibody libraries.Amplification of immunoglobulin variable gene fragments from single B cell clones.
	Working dilution 10 pmol/microliter (Ready-to-use for common PCR).Dilution buffer H2O.Category PCR oligonucleotide primer.
Purity	Purified by HPLC/HPSF.
Specificity	Determination Human IgG and IgM Library Primer Set 1 and 2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.