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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (Biotin)

Anti -IgG, Fc (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
I1904-14B is ideal for investigators involved in Serum Protein Component research.
Catalog #I1904-14B
ApplicationsSuitable for use in Immunomicroscopy, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:20,000-1:130,000. Assayed against 1ug mouse IgG in a standard ELISA using Streptavidin (HRP) (S7973-97A) and ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilution determined by the researcher.
Recommended SecondaryS7973-97A: Streptavidin (HRP)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Thimerisol. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgG F(c) fragment.
SpecificityRecognizes mouse IgG. Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgG, mouse IgG F(c) and mouse serum. No reaction was observed against mouse IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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