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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IF

Catalog #	I1904-14G
Applications	Suitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Label	Fluorescein isothiocyanate (FITC)
	Molecular Weight: 390D
	Absorption Wavelength: 495nm
	Emission Wavelength: 528nm
	Fluorochrome/Protein Ratio: As reported
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label with Fluorescein isothiocyanate (FITC).. Reconstitute with 1ml sterile dH2O or 40-50% glycerol for long term storage.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Mouse IgG F(c) fragment
Specificity	Recognzizes Mouse IgG F(c) fragment. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgG, mouse IgG F(c) and mouse serum. Does not crossreact with anti-pepsin, anti-goat IgG F(c), mouse IgG F(ab’)2 or bovine, horse and human serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.