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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc, X-adsorbed (Biotin)

Anti -IgG, Fc, X-adsorbed (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-14J
ApplicationsSuitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested
Recommended Dilutions
ELISA: 1:4000-1:20,000
Optimal dilution determined by the researcher.
Label Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Companion ProductsA0030: ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO
S7973-97A: Streptavidin (HRP)
Also AvailableI1904-04B: IgG, H&L (Biotin) Pab Gt xMo
I1904-06B: IgG, H&L, X-Adsorbed (Biotin) Pab Gt xMo
I1904-06G: IgG, H&L, X-Adsorbed (Biotin) Pab Gt xMo
I1904-09S: IgG, H&L (Biotin) Pab Gt xMo
I1904-09T: IgG, H&L, X-adsorbed (Biotin) Pab Gt xMo
I1904-09U: IgG, H&L, X-adsorbed (Biotin) Pab Gt xMo
I1904-14B: IgG, Fc (Biotin) Pab Gt xMo
I1904-14J: IgG, Fc, X-adsorbed (Biotin) Pab Gt xMo
I1904-19B: IgG, F(abí)2 (Biotin) Pab Gt xMo
I1904-25B: IgG, IgA, IgM H&L (Biotin) Pab Gt xMo
I1904-35N: IgG, IgA, IgM, H&L, X-adsorbed (Biotin) Pab Gt xMo
I1904-67QIgG F(abí)2, X-adsorbed (Biotin) Pab Gt xMo
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgG F(c) fragment
SpecificityRecognizes Mouse IgG F(c) fragment. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgG, Mouse IgG F(c) and mouse serum. Species Crossreactivity: Does not crossreact with anti-pepsin, anti-goat IgG F(c), mouse IgG F(abí)2 or bovine, equine and human serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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