| Catalog # | I1904-14J |
| Applications | Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested |
| Recommended Dilutions | |
| ELISA: 1:4000-1:20,000 |
| Optimal dilution determined by the researcher. |
| Label | Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) |
| Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Companion Products | A0030: ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO |
| S7973-97A: Streptavidin (HRP) |
| Also Available | I1904-04B: IgG, H&L (Biotin) Pab Gt xMo |
| I1904-06B: IgG, H&L, X-Adsorbed (Biotin) Pab Gt xMo |
| I1904-06G: IgG, H&L, X-Adsorbed (Biotin) Pab Gt xMo |
| I1904-09S: IgG, H&L (Biotin) Pab Gt xMo |
| I1904-09T: IgG, H&L, X-adsorbed (Biotin) Pab Gt xMo |
| I1904-09U: IgG, H&L, X-adsorbed (Biotin) Pab Gt xMo |
| I1904-14B: IgG, Fc (Biotin) Pab Gt xMo |
| I1904-14J: IgG, Fc, X-adsorbed (Biotin) Pab Gt xMo |
| I1904-19B: IgG, F(ab’)2 (Biotin) Pab Gt xMo |
| I1904-25B: IgG, IgA, IgM H&L (Biotin) Pab Gt xMo |
| I1904-35N: IgG, IgA, IgM, H&L, X-adsorbed (Biotin) Pab Gt xMo |
| I1904-67QIgG F(ab’)2, X-adsorbed (Biotin) Pab Gt xMo |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | F(ab’)2 |
| Host | Goat |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Mouse IgG F(c) fragment |
| Specificity | Recognizes Mouse IgG F(c) fragment. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgG, Mouse IgG F(c) and mouse serum. Species Crossreactivity: Does not crossreact with anti-pepsin, anti-goat IgG F(c), mouse IgG F(ab’)2 or bovine, equine and human serum proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
