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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (HRP)

Anti -IgG, Fc (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #I1904-14K
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron
Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:5000-1:12,000 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
SourceMouse
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% Gentamicin Sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 500ul sterile, ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgG F(c).
SpecificityRecognizes mouse IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, mouse IgG, mouse IgG F(c) and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), mouse IgG F(abí)2 or bovine, equine, or human serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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