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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH Gel

Catalog #	I1904-14K
Applications	Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron
	Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	ELISA: 1:5000-1:12,000 30 minutes at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Mouse
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% Gentamicin Sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 500ul sterile, ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Mouse IgG F(c).
Specificity	Recognizes mouse IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, mouse IgG, mouse IgG F(c) and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), mouse IgG F(ab’)2 or bovine, equine, or human serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.