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Anti -IgG, Fc, X-adsorbed (TRITC)
Anti -IgG, Fc, X-adsorbed (TRITC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IF |
|
| Catalog # | I1904-14P | | Applications | Suitable for use in ELISA and Immunofluorescence. Other applications have not been tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Label | Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) | | Absorption Wavelength | 550nm | | Emission Wavelength | 570nm | | Fluorochrome/Protein Ratio | 3.6 moles TRITC per mole of Goat IgG F(ab’)2 | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | F(ab’)2 | | Host | Goat | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free) | | 0.01% (w/v) Sodium Azide. Labeled with TRITC. | | Purity | Purified by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. | | Immunogen | Mouse IgG F(ab’)2 fragment | | Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti- | | Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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