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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (HRP)

Anti -IgG, Fc (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-25K
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:6000-1:50,000. 1ug of canine IgG was used in a standard capture ELISA with ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:100-1:10,000
Immunohistochemistry: 1:500-1:2500
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceCanine
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenCanine IgG F(c) whole molecule
SpecificityRecognizes canine IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, canine IgG, canine IgG F(c) and canine serum. No reaction was observed against canine IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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