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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (HRP)

Anti -IgG, Fc (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-25V
ApplicationsSuitable for use in Immunomicroscopy, ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended DilutionELISA: 1:7000-1:40,000, use 1ug in a standard capture ELISA using ABTS as a substrate for 30 min. at RT.
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceCanine
Concentration~1-2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% gentamicin sulfate, before the addition of glycerol to 40%. Conjugated to horseradish peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenCanine IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, canine IgG, canine IgG F(c) and canine serum. No reaction was observed against canine IgG F(abí)2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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