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Specifications

Clone Host Grade Applications Polyclonal Donkey Affinity Purified E B IP IH

Catalog #	I1904-28F
Applications	Suitable for use in Immunomicroscopy, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.Other applications not tested.
Recommended Dilution	:
	ELISA: 1:600,000
	Western Blot: 1:60,000
	Immunohistochemistry: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio	10–20 Biotin molecules per Donkey IgG molecule
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Donkey
Source	Goat
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Goat IgG whole molecule.
Specificity	Recognizes Goat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-donkey serum, goat IgG and goat serum. No reaction was observed against chicken, guinea pig, equine, hamster, mouse, rabbit and rat serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.