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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG, H&L, X-Adsorbed (Biotin)

Anti -IgG, H&L, X-Adsorbed (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Donkey Affinity Purified E B IP IH
Catalog #I1904-28F
ApplicationsSuitable for use in Immunomicroscopy, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.Other applications not tested.
Recommended Dilution:
ELISA: 1:600,000
Western Blot: 1:60,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio 1020 Biotin molecules per Donkey IgG molecule
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostDonkey
SourceGoat
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenGoat IgG whole molecule.
SpecificityRecognizes Goat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-donkey serum, goat IgG and goat serum. No reaction was observed against chicken, guinea pig, equine, hamster, mouse, rabbit and rat serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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