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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (Biotin)

Anti -IgG, Fc (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-28U
Label Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio 10–20 BAC molecules per Rabbit IgG moleculeApplication(s): immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilution(s)ELISA: 1:20,000 to 1:100,000. Assayed against 1ug of goat IgG in a standard capture ELISA using peroxidase conjugated streptavidin. A working dilution of of the reconstitution concentration is suggested for this product.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceGoat
Concentration~2mg/ml (UV280)
FormSupplied as a liquid in 0.02 M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10 mg/ml bovine serum albumin (BSA) IgG and protease free, 0.01% (w/v) thimerosal, 25% glycerol.
PurityPrepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
ImmunogenGoat IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab’)2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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