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You are here:Home » Antibodies » Abs to IgG Fc » Anti -IgG, Fc (AP)

Anti -IgG, Fc (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH Gel
Catalog #I1904-28W
ApplicationsSuitable for use in Western Blot, Dot Blot, ELISA and Immunhistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:400 to 1:2,800 using 1.0 ug of Goat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilutions to be determined by the researcher.
LabelAlkaline Phosphatase (Calf Intestine) (MW 140kD)
Storage and StabilityStore at 2-8°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. AP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceGoat
Concentration~1mg/ml
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).
PurityPurified by immunoaffinity chromatography.
ImmunogenGoat IgG F(c) fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(abí).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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