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You are here:Home » Antibodies » Abs to IgG F(abí)2 » Anti -IgG, F(ab')2 (Biotin)

Anti -IgG, F(ab')2 (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-29
Label Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio 10Ė20 BAC molecules per Rabbit IgG moleculeApplication(s): immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilution(s) This product has been assayed against 1ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:40,000 to 1:200,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Storage Conditions Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceGoat
Concentration~2mg/ml (UV280)
FormBiotin Conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.01% sodium azide
PurityPrepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
ImmunogenGoat IgG F(abí)2 fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG, Goat IgG F(abí)2 and Goat Serum. No reaction was observed against Goat IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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