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You are here:Home » Antibodies » Abs to IgG X-Adsorbed » Anti -IgG F(ab')2, X-adsorbed (HRP)

Anti -IgG F(ab')2, X-adsorbed (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-29N
ApplicationsSuitable for use in Immunoblot, ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:8000-1:62,000; 1.0ug of Goat IgG was used in a standard capture ELISA with ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceGoat
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Label
PurityPurified by immunoaffinity chromatography.
ImmunogenGoat IgG F(ab')2 fragment
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG, Goat IgG F(ab')2 and Goat Serum. No reaction was observed against Goat IgG F(c) or Human Serum Proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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