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Specifications

Clone Host Grade Applications Polyclonal Donkey Affinity Purified E B IH

Catalog #	I1904-29P
Applications	Suitable for use in Immunoblot, ELISA, immunomicroscopy and immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:8000-1:36,000; 1ug of Goat IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS as a substrate for 30 minutes at RT.
	Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio	10-20 BAC molecules per Donkey IgG Fab molecule
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG Fab
Host	Donkey
Source	Goat
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with biotin.
Reconstitute with 1ml sterile 40-50% glycerol, dH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Goat IgG whole molecule
Specificity	Recognizes goat IgG Fab. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-donkey serum. No reaction was observed against anti-papain or anti-donkey IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.