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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG (FITC)

Anti -IgG (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IF
Catalog #I1904-34R
ApplicationsSuitable for ELISA and Immunoflorescence. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Absorption Wavelength495nm
Emission Wavelength528nm
Fluorochrome/Protein Ratio1.6 moles FITC per mole of Goat IgG
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml 40-50% sterile glycerol, ddH2O.
PurityPurified by immunoaffinity chromatography followed by solid phase adsorption(s) to remove any unwanted reactivities.
ImmunogenHuman IgG gamma chain
SpecificityRecognizes human IgG gamma chain. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, human IgG and human serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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