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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH Gel

Catalog #	I1904-34U
Applications	Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:1000-1:2000, using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT.
	Other applications not tested.
Storage and Stability	May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml (UV280)
Form	Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human IgG g (gamma heavy chain)
Specificity	Assay by IEP resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-goat serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human or human heavy or light chain isotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.