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You are here:Home » Antibodies » Abs to IgG Conjugates » Anti -IgG (AP)

Anti -IgG (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1904-34U
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:2000, using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT.
Other applications not tested.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman IgG g (gamma heavy chain)
SpecificityAssay by IEP resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-goat serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human or human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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