| Catalog # | I1904-34X |
| Applications | Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested. |
| Recommended Dilution | ELISA: 1:20,000-1:100,000 |
| Western Blot: 1:2000-1:10,000 |
| Immunohistochemistry: 1:1000-1:5000 |
| Optimal dilutions to be determined by the researcher. |
| Label | Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) |
| Biotin/Protein Ratio | 10–20 BAC molecules per goat IgG molecule |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~2mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Conjugated to Biotin. |
| Purity | Purified by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
| Immunogen | Human IgG F(c) fragment |
| Specificity | Recognizes human IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F(ab’)2. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
